Eosinophil purification from peripheral blood
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Date
2014
Author(s)
Akuthota, Praveen
Capron, Kelsey
Publisher
Springer Science + Business Media
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Citation
Akuthota, Praveen, Kelsey Capron, and Peter F. Weller. 2014. Eosinophil purification from peripheral blood. Eosinophils: 13–20. doi:10.1007/978-1-4939-1016-8_2.
Abstract
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
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