Publication: Eosinophil purification from peripheral blood
Loading...
Open/View Files
Date
2014
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Science + Business Media
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Akuthota, Praveen, Kelsey Capron, and Peter F. Weller. 2014. Eosinophil purification from peripheral blood. Eosinophils: 13–20. doi:10.1007/978-1-4939-1016-8_2.
Research Data
Abstract
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
Description
Other Available Sources
Keywords
humans, eosinophils, granulocytes, cell separation/instrumentation, cell separation/methods, granulocytes
Terms of Use
This article is made available under the terms and conditions applicable to Open Access Policy Articles (OAP), as set forth at Terms of Service