Publication: Cellular O-Glycome Reporter/Amplification to Explore O-Glycans of Living Cells
Open/View Files
Date
2015
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Kudelka, Matthew R., Aristotelis Antonopoulos, Yingchun Wang, Duc M. Duong, Xuezheng Song, Nicholas T. Seyfried, Anne Dell, Stuart M. Haslam, Richard D. Cummings, and Tongzhong Ju. 2015. “Cellular O-Glycome Reporter/Amplification to Explore O-Glycans of Living Cells.” Nature methods 13 (1): 81-86. doi:10.1038/nmeth.3675. http://dx.doi.org/10.1038/nmeth.3675.
Research Data
Abstract
Protein O-glycosylation plays key roles in many biological processes, but the repertoire of O-glycans synthesized by cells is difficult to determine. Here we describe a new approach termed Cellular O-Glycome Reporter/Amplification (CORA), a sensitive method to amplify and profile mucin-type O-glycans synthesized by living cells. Cells incubated with peracetylated benzyl-α-N-acetylgalactosamine (GalNAc-α-Benzyl) convert it to a large variety of modified O-glycan derivatives that are secreted from cells, allowing easy purification for analysis by HPLC and mass spectrometry (MS). CORA results in ~100–1000-fold increase in sensitivity over conventional O-glycan analyses and identifies a more complex repertoire of O-glycans in more than a dozen cell types from Homo sapiens and Mus musculus. Furthermore, CORA coupled with computational modeling allows predictions on the diversity of the human O-glycome and offers new opportunities to identify novel glycan biomarkers for human diseases.
Description
Other Available Sources
Keywords
Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service