Publication: Cas9 Functionally Opens Chromatin
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Date
2016
Published Version
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Public Library of Science
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Citation
Barkal, Amira A., Sharanya Srinivasan, Tatsunori Hashimoto, David K. Gifford, and Richard I. Sherwood. 2016. “Cas9 Functionally Opens Chromatin.” PLoS ONE 11 (3): e0152683. doi:10.1371/journal.pone.0152683. http://dx.doi.org/10.1371/journal.pone.0152683.
Research Data
Abstract
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
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Keywords
Biology and Life Sciences, Cell Biology, Chromosome Biology, Chromatin, Genetics, Epigenetics, Gene Expression, Medicine and Health Sciences, Clinical Medicine, Clinical Immunology, Hypersensitivity, Immunology, Biology and life sciences, Biochemistry, Proteins, DNA-binding proteins, Nucleases, Deoxyribonucleases, Enzymology, Enzymes, Hydrolases, Molecular Biology, Molecular Biology Techniques, Sequencing Techniques, Sequence Analysis, Sequence Motif Analysis, Genomics, Animal Genomics, Mammalian Genomics, Genetic Loci, Transcription Factors, Gene Regulation, Regulatory Proteins
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