Publication: Two Improved Methods for Preparing Ferritin-Protein Conjugates for Electron Microscopy
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Date
1975-02-01
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Rockefeller University Press
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Kishida, Yasuo, Bjorn Olsen, Richard Berg, Darwin Prockop. "Two Improved Methods for Preparing Ferritin-Protein Conjugates for Electron Microscopy." Journal of Cell Biology 64, no. 2 (1975): 331-339. DOI: 10.1083/jcb.64.2.331
Abstract
Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
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Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biology
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