Publication: Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic
Open/View Files
Date
2018
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Rezanejad, H., L. Ouziel-Yahalom, C. A. Keyzer, B. A. Sullivan, J. Hollister-Lock, W. Li, L. Guo, et al. 2018. “Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic.” Stem Cell Reports 10 (3): 725-738. doi:10.1016/j.stemcr.2018.01.028. http://dx.doi.org/10.1016/j.stemcr.2018.01.028.
Research Data
Abstract
Summary Pancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβhigh/Sox9high) subpopulation was able to form organoids.
Description
Other Available Sources
Keywords
heterogeneity, organoid, pancreatic ductal cells
Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material (LAA), as set forth at Terms of Service