ABIN-1 Regulates RIPK1 Activation by Bridging M1 ubiquitination with K63 Deubiquitination in TNF-RSC

Abstract

Ubiquitination of TNFR1-signaling-complex (TNF-RSC) controls the activation of RIPK1, a kinase critically involved in mediating multiple TNFα activated deleterious events. However, the molecular mechanism that coordinates different types of ubiquitination modifications to regulate the activation of RIPK1 kinase remains unclear. Here, we show that ABIN-1/NAF-1, a ubiquitin-binding protein, is recruited rapidly into TNF-RSC in a manner dependent upon M1 ubiquitinating complex LUBAC to regulate the recruitment of A20 to control K63 deubiquitination of RIPK1. ABIN-1 deficiency reduces the recruitment of A20 and licenses cells to die through necroptosis by promoting K63 ubiquitination and activation of RIPK1 with TNFα stimulation under conditions that would otherwise exclusively activate apoptosis in wild-type cells. Inhibition of RIPK1 kinase and RIPK3 deficiency block the embryonic lethality of Abin-1−/− mice. We propose that ABIN-1 provides a critical link between M1 ubiquitination mediated by LUBAC complex and K63 deubiquitination by phospho-A20 to modulate the activation of RIPK1.