Publication: Cas9 gRNA Engineering for Genome Editing, Activation and Repression
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Date
2015-11
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Springer Science and Business Media LLC
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Citation
Kiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian et al. "Cas9 gRNA Engineering for Genome Editing, Activation and Repression." Nature Methods 12, no. 11 (2015): 1051-1054. DOI: 10.1038/nmeth.3580
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Abstract
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
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Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics
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