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Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR

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2001

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American Society for Microbiology
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Fryer, J. F. L., P. D. Griffiths, J. A. Fishman, V. C. Emery, and D. A. Clark. 2001. “Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR.” Journal of Clinical Microbiology 39 (3) (March 1): 1155–1156. doi:10.1128/jcm.39.3.1155-1156.2001.

Abstract

A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/ g of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.

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