Publication: Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine
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Date
1998-05-01
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American Society for Microbiology
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Akiyoshi, Donna E., Maria Denaro, Haihong Zhu, Julia L. Greenstein, Papia Banderjee, and Jay Fishman. Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine. American Journal of Virology (May 1998): 4503–4507
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Abstract
Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for gag, pol, and env in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attach- ment) region of env. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.
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Keywords
Immunology, Insect Science, Microbiology, Virology
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