Publication: Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila melanogaster
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Date
2008-01
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Springer Nature
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Ni, Jian-Quan, Michele Markstein, Richard Binari, Barret D. Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker et al. "Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila melanogaster." Nature Methods 5, no. 1 (2008): 49-51. DOI: 10.1038/nmeth1146
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Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs have been inserted into the genome randomly using P-element–mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
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Research Subject Categories::NATURAL SCIENCES::Chemistry::Biochemistry::Functional genomics
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