Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction

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Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction

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Title: Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction
Author: Wallace, Stephen; Balskus, Emily Patricia

Note: Order does not necessarily reflect citation order of authors.

Citation: Wallace, Stephen, and Emily P. Balskus. 2015. “Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction.” Angewandte Chemie International Edition 54 (24) (April 29): 7106–7109. doi:10.1002/anie.201502185.
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Abstract: Introducing new reactivity into living organisms is a major challenge in synthetic biology. Despite an increasing interest in both developing aqueous-compatible small molecule catalysts and engineering enzymes to perform new chemistry in vitro, the integration of non-native reactivity into metabolic pathways for small molecule production has been underexplored. Herein we report a biocompatible iron(III) phthalocyanine catalyst capable of efficient olefin cyclopropanation in the presence of a living microorganism. By interfacing this catalyst with E. coli engineered to produce styrene, we synthesize non-natural phenyl cyclopropanes directly from D-glucose in single-vessel fermentations. This process represents the first combination of non-biological carbene-transfer reactivity with cellular metabolism for small molecule production.
Published Version: doi:10.1002/anie.201502185
Other Sources: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494747/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:33445941
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