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Seq-Well: A Portable, Low-Cost Platform for High-Throughput Single-Cell RNA-Seq of Low-Input Samples

 
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Author
Gierahn, Todd M.
Wadsworth, Marc H.
Hughes, Travis K.HARVARD
Bryson, Bryan D.HARVARD
Butler, Andrew
Satija, Rahul
Fortune, SarahHARVARD
Love, J. Christopher
Shalek, Alex K.
Published Version
https://doi.org/10.1038/nmeth.4179
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Citation
Gierahn, Todd M., Marc H. Wadsworth, Travis K. Hughes, Bryan D. Bryson, Andrew Butler, Rahul Satija, Sarah Fortune, J. Christopher Love, and Alex K. Shalek. 2017. “Seq-Well: A Portable, Low-Cost Platform for High-Throughput Single-Cell RNA-Seq of Low-Input Samples.” Nature methods 14 (4): 395-398. doi:10.1038/nmeth.4179. http://dx.doi.org/10.1038/nmeth.4179.
Abstract
Single-cell RNA-Seq can precisely resolve cellular states but application to sparse samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively-parallel single-cell RNA-Seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semi-permeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well using species-mixing experiments and PBMCs, and profile thousands of primary human macrophages exposed to tuberculosis.
Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376227/pdf/
Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:34375305

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