Efficient proximity labeling in living cells and organisms with TurboID
Author
Branon, Tess C
Sanchez, Ariana D
Svinkina, Tanya
Carr, Steven A.
Feldman, Jessica L
Ting, Alice Y.
Published Version
https://doi.org/10.1038/nbt.4201Metadata
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Branon, Tess C, Justin Bosch, Ariana D Sanchez, Namrata Udeshi, Tanya Svinkina, Steven A. Carr, Jessica L Feldman et al. "Efficient proximity labeling in living cells and organisms with TurboID." Nature Biotechnology 36, no. 9 (2018): 880-887. DOI: 10.1038/nbt.4201Abstract
Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 hour labeling times or utilize chemicals with limited cell permeability or high toxicity. We used yeast display-based directed evolution to engineer two promiscuous mutants of biotin ligase, TurboID and miniTurbo, which catalyze PL with much greater efficiency than BioID or BioID2, and enable 10-minute PL in cells with non-toxic and easily deliverable biotin. Furthermore, TurboID extends biotin-based PL to flies and worms.Other Sources
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126969/Terms of Use
This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAPCitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37371714
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