The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
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Perkins, Lizabeth
Holderbaum, Laura
Tao, Rong
Hu, Yanhui
Sopko, Richelle
McCall, Kim
Yang-Zhou, Donghui
Flockhart, Ian
Binari, Richard
Shim, Hye-Seok
Miller, Audrey
Housden, Amy
Foos, Marianna
Randkelv, Sakara
Kelley, Colleen
Namgyal, Pema
Villalta, Christians
Liu, Lu-Ping
Jiang, Xia
Huan-Huan, Qiao
Wang, Xia
Fujiyama, Asao
Toyoda, Atsushi
Ayers, Kathleen
Blum, Allison
Czech, Benjamin
Neumuller, Ralph
Yan, Dong
Cavallaro, Amanda
Hibbard, Karen
Hall, Don
Cooley, Lynn
Hannon, Gregory
Lehmann, Ruth
Parks, Annette
Mohr, Stephanie
Ueda, Ryu
Kondo, Shu
Ni, Jian-Quan
Perrimon, Norbert
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https://doi.org/10.1534/genetics.115.180208Metadata
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Perkins, Lizabeth A., Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, et al. 2015. “The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.” Genetics 201 (3): 843–52. https://doi.org/10.1534/genetics.115.180208.Abstract
To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41384481
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