Intestinal Upregulation of Melanin-Concentrating Hormone in TNBS-Induced Enterocolitis in Adult Zebrafish
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Geiger, Brenda M.
Gras-Miralles, Beatriz
Ziogas, Dimitrios C.
Karagiannis, Apostolos K. A.
Zhen, Aileen
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https://doi.org/10.1371/journal.pone.0083194Metadata
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Geiger, Brenda M., Beatriz Gras-Miralles, Dimitrios C. Ziogas, Apostolos K. A. Karagiannis, Aileen Zhen, Paula Fraenkel, and Efi Kokkotou. 2013. “Intestinal Upregulation of Melanin-Concentrating Hormone in TNBS-Induced Enterocolitis in Adult Zebrafish.” PLoS ONE 8 (12): e83194. doi:10.1371/journal.pone.0083194. http://dx.doi.org/10.1371/journal.pone.0083194.Abstract
Background: Melanin-concentrating hormone (MCH), an evolutionarily conserved appetite-regulating neuropeptide, has been recently implicated in the pathogenesis of inflammatory bowel disease (IBD). Expression of MCH is upregulated in inflamed intestinal mucosa in humans with colitis and MCH-deficient mice treated with trinitrobenzene-sulfonic acid (TNBS) develop an attenuated form of colitis compared to wild type animals. Zebrafish have emerged as a new animal model of IBD, although the majority of the reported studies concern zebrafish larvae. Regulation MCH expression in the adult zebrafish intestine remains unknown. Methods: In the present study we induced enterocolitis in adult zebrafish by intrarectal administration of TNBS. Follow-up included survival analysis, histological assessment of changes in intestinal architecture, and assessment of intestinal infiltration by myeloperoxidase positive cells and cytokine transcript levels. Results: Treatment with TNBS dose-dependently reduced fish survival. This response required the presence of an intact microbiome, since fish pre-treated with vancomycin developed less severe enterocolitis. At 6 hours post-challenge, we detected a significant influx of myeloperoxidase positive cells in the intestine and upregulation of both proinflammatory and anti-inflammatory cytokines. Most importantly, and in analogy to human IBD and TNBS-induced mouse experimental colitis, we found increased intestinal expression of MCH and its receptor in TNBS-treated zebrafish. Conclusions: Taken together these findings not only establish a model of chemically-induced experimental enterocolitis in adult zebrafish, but point to effects of MCH in intestinal inflammation that are conserved across species.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869761/pdf/Terms of Use
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