dc.contributor.author | Geiger, Brenda M. | en_US |
dc.contributor.author | Gras-Miralles, Beatriz | en_US |
dc.contributor.author | Ziogas, Dimitrios C. | en_US |
dc.contributor.author | Karagiannis, Apostolos K. A. | en_US |
dc.contributor.author | Zhen, Aileen | en_US |
dc.contributor.author | Fraenkel, Paula | en_US |
dc.contributor.author | Kokkotou, Efi | en_US |
dc.date.accessioned | 2014-03-11T10:15:19Z | |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Geiger, Brenda M., Beatriz Gras-Miralles, Dimitrios C. Ziogas, Apostolos K. A. Karagiannis, Aileen Zhen, Paula Fraenkel, and Efi Kokkotou. 2013. “Intestinal Upregulation of Melanin-Concentrating Hormone in TNBS-Induced Enterocolitis in Adult Zebrafish.” PLoS ONE 8 (12): e83194. doi:10.1371/journal.pone.0083194. http://dx.doi.org/10.1371/journal.pone.0083194. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879198 | |
dc.description.abstract | Background: Melanin-concentrating hormone (MCH), an evolutionarily conserved appetite-regulating neuropeptide, has been recently implicated in the pathogenesis of inflammatory bowel disease (IBD). Expression of MCH is upregulated in inflamed intestinal mucosa in humans with colitis and MCH-deficient mice treated with trinitrobenzene-sulfonic acid (TNBS) develop an attenuated form of colitis compared to wild type animals. Zebrafish have emerged as a new animal model of IBD, although the majority of the reported studies concern zebrafish larvae. Regulation MCH expression in the adult zebrafish intestine remains unknown. Methods: In the present study we induced enterocolitis in adult zebrafish by intrarectal administration of TNBS. Follow-up included survival analysis, histological assessment of changes in intestinal architecture, and assessment of intestinal infiltration by myeloperoxidase positive cells and cytokine transcript levels. Results: Treatment with TNBS dose-dependently reduced fish survival. This response required the presence of an intact microbiome, since fish pre-treated with vancomycin developed less severe enterocolitis. At 6 hours post-challenge, we detected a significant influx of myeloperoxidase positive cells in the intestine and upregulation of both proinflammatory and anti-inflammatory cytokines. Most importantly, and in analogy to human IBD and TNBS-induced mouse experimental colitis, we found increased intestinal expression of MCH and its receptor in TNBS-treated zebrafish. Conclusions: Taken together these findings not only establish a model of chemically-induced experimental enterocolitis in adult zebrafish, but point to effects of MCH in intestinal inflammation that are conserved across species. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0083194 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869761/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Intestinal Upregulation of Melanin-Concentrating Hormone in TNBS-Induced Enterocolitis in Adult Zebrafish | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Fraenkel, Paula | en_US |
dc.date.available | 2014-03-11T10:15:19Z | |
dc.identifier.doi | 10.1371/journal.pone.0083194 | * |
dash.contributor.affiliated | Fraenkel, Paula | |
dash.contributor.affiliated | Kokkotou, Efi | |