Rapid De-Localization of Actin Leading Edge Components with BDM Treatment

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Rapid De-Localization of Actin Leading Edge Components with BDM Treatment

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dc.contributor.author Yarrow, Justin C
dc.contributor.author Lechler, Terry
dc.contributor.author Li, Rong
dc.contributor.author Mitchison, Timothy J.
dc.date.accessioned 2011-03-27T20:32:51Z
dc.date.issued 2003
dc.identifier.citation Yarrow, Justin C., Terry Lechler, Rong Li, and Timothy J. Mitchison. 2003. Rapid de-localization of actin leading edge components with BDM treatment. BMC Cell Biology 4: 5. en_US
dc.identifier.issn 1471-2121 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4774194
dc.description.abstract Background: 2,3-Butanedione monoxime (BDM) has been widely used as a non-muscle myosin inhibitor to investigate the role of non-muscle myosinII in the process of actin retrograde flow and other actin cytoskeletal processes. Recent reports show that BDM does not inhibit any non-muscle myosins so far tested, including nm-myosinII, prompting the question, how were these process affected in BDM studies? Results: We have found that treatment of mammalian cells with BDM for only 1 min blocks actin incorporation at the leading edge in a permeabilized cell system. We show that inhibition of actin incorporation occurs through de-localization of leading edge proteins involved in actin polymerization – the Arp2/3 complex, WAVE, and VASP – that de-localize concomitantly with the leading edge actin network. Conclusion: De-localization of actin leading edge components by BDM treatment is a newly described effect of this compound. It may explain many of the results previously ascribed to inhibition of non-muscle myosinII by BDM, particularly in studies of leading edge dynamics. Though this effect of BDM is intriguing, future studies probing actin dynamics at the leading edge should use more potent and specific inhibitors. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2121-4-5 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165424/pdf/ en_US
dash.license LAA
dc.title Rapid De-Localization of Actin Leading Edge Components with BDM Treatment en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Cell Biology en_US
dash.depositing.author Mitchison, Timothy J.
dc.date.available 2011-03-27T20:32:51Z
dash.affiliation.other HMS^Systems Biology en_US

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