Browsing by Author "Bershtein, Shimon"
Now showing items 1-7 of 7
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Biophysical principles predict fitness landscapes of drug resistance
Rodrigues, Joao Vierira; Bershtein, Shimon; Li, Annabel; Lozovsky, Elena; Hartl, Daniel L.; Shakhnovich, Eugene Isaacovitch (Proceedings of the National Academy of Sciences, 2016)Fitness landscapes of drug resistance constitute powerful tools to elucidate mutational pathways of antibiotic escape. Here, we de- veloped a predictive biophysics-based fitness landscape of trimeth- oprim (TMP) resistance ... -
Chromosomal Barcoding of E. Coli Populations Reveals Lineage Diversity Dynamics at High Resolution
Jasinska, Weronika; Manhart, Michael; Lerner, Jesse; Gauthier, Louis; Serohijos, Adrian; Bershtein, Shimon (Nature Publishing Group, 2020-03)Evolutionary dynamics in large asexual populations is strongly influenced by multiple competing beneficial lineages, most of which segregate at very low frequencies. However, technical barriers to tracking a large number ... -
Protein Homeostasis Imposes a Barrier on Functional Integration of Horizontally Transferred Genes in Bacteria
Bershtein, Shimon; Serohijos, Adrian W. R.; Bhattacharyya, Sanchari; Manhart, Michael; Choi, Jeong-Mo; Mu, Wanmeng; Zhou, Jingwen; Shakhnovich, Eugene I. (Public Library of Science, 2015)Horizontal gene transfer (HGT) plays a central role in bacterial evolution, yet the molecular and cellular constraints on functional integration of the foreign genes are poorly understood. Here we performed inter-species ... -
Protein Homeostasis Imposes a Barrier on Functional Integration of Horizontally Transferred Genes in Bacteria
Bershtein, Shimon; Serohijos, Adrian W. R.; Bhattacharyya, Sanchari; Manhart, Michael; Choi, Jeong-Mo; Mu, Wanmeng; Zhou, Jingwen; Shakhnovich, Eugene I. (Public Library of Science, 2015)Horizontal gene transfer (HGT) plays a central role in bacterial evolution, yet the molecular and cellular constraints on functional integration of the foreign genes are poorly understood. Here we performed inter-species ... -
Protein Quality Control Acts on Folding Intermediates to Shape the Effects of Mutations on Organismal Fitness
Bershtein, Shimon; Mu, Wanmeng; Serohijos, Adrian; Zhou, Jingwen; Shakhnovich, Eugene Isaacovitch (Elsevier BV, 2012)What are the molecular properties of proteins that fall on the radar of protein quality control (PQC)? Here we mutate the E. coli’s gene encoding dihydrofolate reductase (DHFR) and replace it with bacterial orthologous ... -
Soluble oligomerization provides a beneficial fitness effect on destabilizing mutations
Bershtein, Shimon; Mu, W.; Shakhnovich, Eugene Isaacovitch (Proceedings of the National Academy of Sciences, 2012)Mutations create the genetic diversity on which selective pressures can act, yet also create structural instability in proteins. How, then, is it possible for organisms to ameliorate mutation-induced perturbations of protein ... -
Systems-Level Response to Point Mutations in a Core Metabolic Enzyme Modulates Genotype-Phenotype Relationship
Bershtein, Shimon; Choi, Jeong-Mo; Bhattacharyya, Sanchari; Budnik, Bogdan; Shakhnovich, Eugene Isaacovitch (Elsevier BV, 2015)Linking the molecular effects of mutations to fitness is central to understanding evolutionary dynamics. Here we establish a quantitative relation between the global effect of mutations on the E. coli proteome and bacterial ...