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Li, Pulin

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Li

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Pulin

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Li, Pulin

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2010 - 20112

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Author's Publications: search.filters.isAuthorOfPublication.4625171c-417f-41ee-b067-1405a20dadfb

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    Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish
    (The Company of Biologists, 2010) Mosimann, Christian; Kaufman, Charles; Li, Pulin; Pugach, Emily K.; Tamplin, Owen J.; Zon, Leonard
    Molecular genetics approaches in zebrafish research are hampered by the lack of a ubiquitous transgene driver element that is active at all developmental stages. Here, we report the isolation and characterization of the zebrafish ubiquitin (ubi) promoter, which drives constitutive transgene expression during all developmental stages and analyzed adult organs. Notably, ubi expresses in all blood cell lineages, and we demonstrate the application of ubi-driven fluorophore transgenics in hematopoietic transplantation experiments to assess true multilineage potential of engrafted cells. We further generated transgenic zebrafish that express ubiquitous 4-hydroxytamoxifen-controlled Cre recombinase activity from a \(ubi:cre^{ERt2}\) transgene, as well as ubi:loxP-EGFP-loxP-mCherry (ubi:Switch) transgenics and show their use as a constitutive fluorescent lineage tracing reagent. The ubi promoter and the transgenic lines presented here thus provide a broad resource and important advancement for transgenic applications in zebrafish.
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    Lineage Regulators Direct BMP and Wnt Pathways to Cell-Specific Programs during Differentiation and Regeneration
    (Elsevier BV, 2011) Trompouki, Eirini; Bowman, Teresa Venezia; Lawton, Lee N.; Fan, Zi Peng; Wu, Dai-Chen; DiBiase, Anthony; Martin, Corey S.; Cech, Jennifer N.; Sessa, Anna K.; Leblanc, Jocelyn L.; Li, Pulin; Durand, Ellen; Mosimann, Christian; Heffner, Garrett C.; Daley, George; Paulson, Robert F.; Young, Richard A.; Zon, Leonard
    BMP and Wnt signaling pathways control essential cellular responses through activation of the transcription factors SMAD (BMP) and TCF (Wnt). Here, we show that regeneration of hematopoietic lineages following acute injury depends on the activation of each of these signaling pathways to induce expression of key blood genes. Both SMAD1 and TCF7L2 co-occupy sites with master regulators adjacent to hematopoietic genes. In addition, both SMAD1 and TCF7L2 follow the binding of the predominant lineage regulator during differentiation from multipotent hematopoietic progenitor cells to erythroid cells. Furthermore, induction of the myeloid lineage regulator C/EBPα in erythroid cells shifts binding of SMAD1 to sites newly occupied by C/EBPα, whereas expression of the erythroid regulator GATA1 directs SMAD1 loss on nonerythroid targets. We conclude that the regenerative response mediated by BMP and Wnt signaling pathways is coupled with the lineage master regulators to control the gene programs defining cellular identity.