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Reátegui, Eduardo

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Reátegui

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Eduardo

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Reátegui, Eduardo

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2016 - 20172

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Author's Publications: search.filters.isAuthorOfPublication.7220efb0-e69a-462c-a70f-5d082c18e23c

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    Porous microwells for geometry-selective, large-scale microparticle arrays
    (2016) Kim, Jae Jung; Bong, Ki Wan; Reátegui, Eduardo; Irimia, Daniel; Doyle, Patrick S.
    Large-scale microparticle arrays (LSMA) are key for material science and bioengineering applications. However, previous approaches suffer from tradeoffs between scalability, precision, specificity, and versatility. Here, we present a porous microwell-based approach to create large-scale microparticle arrays with complex motifs. Microparticles are guided to and pushed into microwells by fluid flow through small open pores at the bottom of the porous well arrays. A scaling theory allows for the rational design of LSMAs to sort and array particles based on their size, shape or modulus. Sequential particle assembly allows for proximal and nested particle arrangements, as well as particle recollection and pattern transfer. We demonstrate the capabilities of the approach by means of three applications: high-throughput single-cell arrays; microenvironment fabrication for neutrophil chemotaxis; and complex, covert tags by the transfer of an upconversion nanocrystal laden LSMA.
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    Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming
    (2017) Reátegui, Eduardo; Jalali, Fatemeh; Khankhel, Aimal H.; Wong, Elisabeth; Cho, Hansang; Lee, Jarone; Serhan, Charles; Dalli, Jesmond; Elliott, Hunter; Irimia, Daniel
    Neutrophil swarms protect healthy tissues by sealing off sites of infection. In the absence of swarming, microbial invasion of surrounding tissues can result in severe infections. Recent observations in animal models have shown that swarming requires rapid neutrophil responses and well-choreographed neutrophil migration patterns. However, in animal models physical access to the molecular signals coordinating neutrophil activities during swarming is limited. Here, we report the development and validation of large microscale arrays of zymosan-particle clusters for the study of human neutrophils during swarming ex vivo. We characterized the synchronized swarming of human neutrophils under the guidance of neutrophil-released chemokines, and measured the mediators released at different phases of human-neutrophil swarming against targets simulating infections. We found that the network of mediators coordinating human-neutrophil swarming includes start and stop signals, proteolytic enzymes and enzyme inhibitors, as well as modulators of activation of other immune and non-immune cells. We also show that the swarming behavior of neutrophils from patients following major trauma is deficient and gives rise to smaller swarms than those of neutrophils from healthy individuals.