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Daane, Jacob

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Daane

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Jacob

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Daane, Jacob
Author's Publications: search.filters.isAuthorOfPublication.ea4ec2ad-6998-41d3-bdf7-326438a64694

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    Bioelectric Signaling Regulates Size in Zebrafish Fins
    (Public Library of Science, 2014) Perathoner, Simon; Daane, Jacob; Henrion, Ulrike; Seebohm, Guiscard; Higdon, Charles W.; Johnson, Stephen L.; Nüsslein-Volhard, Christiane; Harris, Matthew
    The scaling relationship between the size of an appendage or organ and that of the body as a whole is tightly regulated during animal development. If a structure grows at a different rate than the rest of the body, this process is termed allometric growth. The zebrafish another longfin (alf) mutant shows allometric growth resulting in proportionally enlarged fins and barbels. We took advantage of this mutant to study the regulation of size in vertebrates. Here, we show that alf mutants carry gain-of-function mutations in kcnk5b, a gene encoding a two-pore domain potassium (K+) channel. Electrophysiological analysis in Xenopus oocytes reveals that these mutations cause an increase in K+ conductance of the channel and lead to hyperpolarization of the cell. Further, somatic transgenesis experiments indicate that kcnk5b acts locally within the mesenchyme of fins and barbels to specify appendage size. Finally, we show that the channel requires the ability to conduct K+ ions to increase the size of these structures. Our results provide evidence for a role of bioelectric signaling through K+ channels in the regulation of allometric scaling and coordination of growth in the zebrafish.
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    Historical Contingency Shapes Adaptive Radiation in Antarctic Fishes
    (Springer Science and Business Media LLC, 2019-06-10) Smits, Patrick; Daane, Jacob; Dornburg, Alex; MacGuigan, Daniel; Hawkins, Brent; Near, Thomas; Detrich, William; Harris, Matthew
    Adaptive radiation illustrates links between ecological opportunity, natural selection, and the generation of biodiversity. Central to adaptive radiation is the association between a diversifying lineage and the evolution of phenotypic variation that facilitates the utilization of novel environments or resources. However, is not clear whether adaptive evolution or historical contingency is more important for the origin of key phenotypic traits in adaptive radiation. Here we use targeted sequencing of >250,000 loci across 46 species to examine hypotheses concerning the origin and diversification of key traits in the adaptive radiation of Antarctic notothenioid fishes. Contrary to expectations of adaptive evolution, we show that notothenioids experienced a punctuated burst of genomic diversification and evolved key skeletal modifications before the onset of polar conditions in the Southern Ocean. We show that diversifying selection in pathways associated with human skeletal dysplasias facilitates ecologically important variation in buoyancy among Antarctic notothenioid species, and demonstrate the sufficiency of altered trip11, col1a2 and col1a1 function in zebrafish (Danio rerio) to phenocopy skeletal reduction in Antarctic notothenioids. Rather than adaptation being driven by the cooling of the Antarctic, our results highlight the role of historical contingency in shaping the adaptive radiation of notothenioids. Understanding the historical and environmental context for the origin of key traits in adaptive radiations extends beyond reconstructing events that result in evolutionary innovation as it also provides a context in forecasting the effects of climate change on the stability and evolvability of natural populations.