Person: Xu, Feng
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Publication Morphological and molecular identification of a new species of Truncospora (Polyporales, Basidiomycota) in North America(Magnolia Press, 2016) ZHAO, CHANG-LIN; Xu, Feng; Pfister, DonaldTruncospora wisconsinensis sp. nov., a new poroid wood-inhabiting species, is proposed based on a combination of molecular and morphological data. This species demonstrates a unique combination of characters including: annual habit; pileate basidiomata with a white pileus and pore surface; a dimitic hyphal system with non- to slightly dextrinoid, cyanophilous skeletal hyphae; and ellipsoid, truncate, slightly thick-walled, strongly dextrinoid basidiospores. Phylogenetic analyses using ITS and partial tef1-α support the position of this new species as a sister clade of T. ohiensis.Publication Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds(Public Library of Science, 2011) Xu, Feng; Sridharan, BanuPriya; Durmus, Naside Gozde; Wang, ShuQi; Yavuz, Ahmet Sinan; Gurkan, Umut; Demirci, UtkanDecellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types.Publication Blood Banking in Living Droplets(Public Library of Science, 2011) Samot, Josh; Moon, Sangjun; Shao, Lei; Zhang, Xiaohui; Xu, Feng; Song, YoungSeok; Keles, Hasan Onur; Matloff, Laura; Markel, Jordan; Demirci, UtkanBlood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional cryopreservation techniques, it has not been incorporated into transfusion medicine mainly due to throughput challenges. Here, we present a scalable method that can vitrify red blood cells in microdroplets. This approach enables the vitrification of large volumes of blood in a short amount of time, and makes it a viable and scalable biotechnology tool for blood cryopreservation.