Publication: Sequencing by Hybridization of Long Targets
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Date
2012
Published Version
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Public Library of Science
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Citation
Qin, Yu, Tobias M. Schneider, and Michael P. Brenner. 2012. Sequencing by hybridization of long targets. PLoS ONE 7(5): e35819.
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Abstract
Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined \(l\)-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to \(n=O(2^{l})\) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.
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Keywords
biology, computational biology, genomics, genome sequencing, sequence analysis, genome analysis tools, sequence assembly tools, theoretical biology, mathematics, applied mathematics, algorithms
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