Browsing by Author "O'Shea, Erin"
Now showing items 1-16 of 16
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The anticancer natural product ophiobolin A induces cytotoxicity by covalent modification of phosphatidylethanolamine
Chidley, Christopher; Trauger, Sunia A; Birsoy, Kıvanç; O'Shea, Erin K (eLife Sciences Publications, Ltd, 2016)Phenotypic screens allow the identification of small molecules with promising anticancer activity, but the difficulty in characterizing the mechanism of action of these compounds in human cells often undermines their value ... -
Circadian Gene Expression in Cyanobacteria
Vijayan, Vikram (2013-03-18)Cyanobacteria are photosynthetic prokaryotes that live in aquatic environments. The cyanobacterium Synechococcus elongatus PCC 7942, (hereafter S. elongatus) coordinates its day and night behaviors via a circadian clock. ... -
Genome-Wide Characterization of the Phosphate Starvation Response in Schizosaccharomyces pombe
Carter-O'Connell, Ian; Peel, Michael T.; Wykoff, Dennis D.; O'Shea, Erin K. (Springer Science + Business Media, 2012)Background: Inorganic phosphate is an essential nutrient required by organisms for growth. During phosphate starvation, Saccharomyces cerevisiae activates the phosphate signal transduction (PHO) pathway, leading to expression ... -
A High Resolution Map of a Cyanobacterial Transcriptome
Vijayan, Vikram; Jain, Isha Himani; O'Shea, Erin K (BioMed Central, 2012)Background: Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform ... -
Hog1 Controls Global Reallocation of RNA Pol II upon Osmotic Shock in Saccharomyces cerevisiae
Cook, Kristen; O'Shea, Erin K (Genetics Society of America, 2012)When challenged with osmotic shock, Saccharomyces cerevisiae induces hundreds of genes, despite a concurrent reduction in overall transcriptional capacity. The stress-responsive MAP kinase Hog1 activates expression of ... -
The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
Grimes, Catherine Leimkuhler; Ariyananda, Lushanti De Zoysa; Melnyk, James E.; O'Shea, Erin K (American Chemical Society, 2012)Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn’s disease, Blau syndrome, and gastrointestinal cancers. ... -
The Molecular Clockwork of a Protein-Based Circadian Oscillator
Markson, Joseph Scott; O'Shea, Erin K (Elsevier, 2009)The circadian clock of the cyanobacterium Synechococcuselongatus PCC 7942 is governed by a core oscillator consisting of the proteins KaiA, KaiB, and KaiC. Remarkably, circadian oscillations in the phosphorylation state ... -
Oscillations in Supercoiling Drive Circadian Gene Expression in Cyanobacteria
Vijayan, Vikram; Zuzow, Rick; O'Shea, Erin K (National Academy of Sciences, 2009)The cyanobacterium Synechococcus elongatus PCC 7942 exhibits oscillations in mRNA transcript abundance with 24-h periodicity under continuous light conditions. The mechanism underlying these oscillations remains elusive—neither ... -
Promoter Decoding of Transcription Factor Translocation Dynamics
Hansen, Anders Sejr (2015-05-11)Many cellular signaling pathways exhibit a bowtie topology: multiple distinct signal inputs converge on a single master transcription factor, which controls the expression of downstream genes. Recent evidence suggests that ... -
Regulation of Genome-Wide Transcriptional Stress Responses in Saccharomyces cerevisiae
Cook, Kristen (2013-01-02)In response to osmotic shock in Saccharomyces cerevisiae the MAP kinase Hog1 coordinates a large-scale transcriptional stress response, rapidly producing hundreds of copies of specified transcripts. Many of the most highly ... -
Robust Circadian Oscillations in Growing Cyanobacteria Require Transcriptional Feedback
Teng, Shu-Wen; Mukherji, Shankar Shankar; Moffitt, Jeffrey R; de Buyl, Sophie; O'Shea, Erin K (American Association for the Advancement of Science, 2013)The remarkably stable circadian oscillations of single cyanobacteria enable a population of growing cells to maintain synchrony for weeks. The cyanobacterial pacemaker is a posttranslational regulation (PTR) circuit that ... -
Sensing Inorganic Phosphate Starvation by the Phosphate-Responsive (PHO) Signaling Pathway of Saccharomyces cerevisiae
Choi, Joonhyuk (2013-09-30)Inorganic phosphate \((P_i)\) is an essential nutrient whose intracellular levels are maintained by the PHO pathway in Saccharomyces cerevisiae. \(P_i\) limitation triggers upregulation of the PHO genes whose gene products ... -
Sequence Determinants of Circadian Gene Expression Phase in Cyanobacteria
Vijayan, Vikram; O'Shea, Erin K. (American Society for Microbiology, 2012)The cyanobacterium Synechococcus elongatus PCC 7942 exhibits global biphasic circadian oscillations in gene expression under constant-light conditions. Class I genes are maximally expressed in the subjective dusk, whereas ... -
Structural Analysis of the CDK-Cyclin Complex of Pho85-Pho80 and Genome-Wide Characterization of the Phosphate Starvation Response in Schizosaccharomyces pombe
Carter-O'Connell, Ian O’Brien (2012-08-17)Inorganic phosphate is an essential nutrient required by all organisms for optimal growth. During phosphate starvation, Saccharomyces cerevisiae induces a set of genes responsible for the regulation of inorganic phosphate ... -
Systems Level Studies of Nutrient Homeostasis
Rizvi, Abbas (2013-02-25)In conditions of phosphate deprivation, the budding yeast, Saccharomyces cerevisiae activates the phosphate starvation response pathway (PHO pathway). Induction of the PHO pathway causes the transcription of genes involved ... -
Two Antagonistic Clock-Regulated Histidine Kinases Time the Activation of Circadian Gene Expression
Gutu, Andrian; O'Shea, Erin K. (Elsevier BV, 2013)The cyanobacterial circadian pacemaker consists of a three-protein clock—KaiA, KaiB, and KaiC—that generates oscillations in the phosphorylation state of KaiC. Here we investigate how temporal information encoded in KaiC ...