Browsing by Author "Huttlin, Edward"

Now showing items 1-4 of 4

    • Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization 

      Sarraf, Shireen A.; Raman, Malavika; Guarani-Pereira, Virginia; Sowa, Mathew E.; Huttlin, Edward L.; Gygi, Steven P.; Harper, J. Wade (2013)
      The PARKIN (PARK2) ubiquitin ligase and its regulatory kinase PINK1 (PARK6), often mutated in familial early onset Parkinson’s Disease (PD), play central roles in mitochondrial homeostasis and mitophagy.1–3 While PARKIN ...

    • A multi-scale map of cell structure fusing protein images and interactions 

      Qin, Yue; Huttlin, Edward; Winsnes, Casper F.; Gosztyla, Maya L.; Wacheul, Ludivine; Kelly, Marcus R.; Blue, Steven M.; Zheng, Fan; Chen, Michael; Schaffer, Leah V.; Licon, Katherine; Bäckström, Anna; Vaites, Laura Pontano; Lee, John J.; Ouyang, Wei; Liu, Sophie N.; Zhang, Tian; Silva, Erica; Park, Jisoo; Pitea, Adriana; Kreisberg, Jason F.; Gygi, Steven P.; Ma, Jianzhu; Harper, J. Wade; Yeo, Gene W.; Lafontaine, Denis L. J.; Lundberg, Emma; Ideker, Trey (Springer Science and Business Media LLC, 2021-11-24)
      The eukaryotic cell is a multi-scale structure with modular organization across at least four orders of magnitude<jats:sup>1,2</jats:sup>. Two central approaches for mapping this structure – protein fluorescent imaging and ...

    • MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes 

      McAlister, Graeme C.; Nusinow, David P.; Jedrychowski, Mark P.; Wühr, Martin; Huttlin, Edward L.; Erickson, Brian K.; Rad, Ramin; Haas, Wilhelm; Gygi, Steven P. (American Chemical Society, 2014)
      Multiplexed quantitation via isobaric chemical tags (e.g., tandem mass tags (TMT) and isobaric tags for relative and absolute quantitation (iTRAQ)) has the potential to revolutionize quantitative proteomics. However, until ...

    • An ultra-tolerant database search reveals that a myriad of modified peptides contributes to unassigned spectra in shotgun proteomics 

      Chick, Joel M.; Kolippakkam, Deepak; Nusinow, David P.; Zhai, Bo; Rad, Ramin; Huttlin, Edward L.; Gygi, Steven P. (2015)
      Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using ...