Browsing by Author "Lajoie, Marc"

Now showing items 1-6 of 6

    • Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection 

      Carr, Peter A.; Wang, Harris He; Sterling, Bram; Isaacs, Farren J.; Lajoie, Marc Joseph; Xu, George Jing; Church, George McDonald; Jacobson, Joseph M. (Oxford University Press, 2012)
      Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded ...

    • Genome Engineering Technologies to Change the Genetic Code 

      Lajoie, Marc Joseph (2014-02-25)
      New technologies are making it possible to engineer organisms with fundamentally new and useful properties. In vivo genome engineering technologies capable of manipulating genomes from the nucleotide to the megabase scale ...

    • Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases 

      Mosberg, Joshua Adam Weintrob; Gregg, Christopher; Lajoie, Marc Joseph; Wang, Harris He; Church, George McDonald (Public Library of Science, 2012)
      Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous ...

    • Manipulating Replisome Dynamics to Enhance Lambda Red-Mediated Multiplex Genome Engineering 

      Lajoie, Marc Joseph; Gregg, Christopher; Mosberg, Joshua Adam Weintrob; Washington, G. C.; Church, George McDonald (Oxford University Press, 2012)
      Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the ...

    • Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria 

      Ling, Jiqiang; Daoud, Rachid; Lajoie, Marc J.; Church, George M.; Söll, Dieter; Lang, B. Franz (Oxford University Press, 2013)
      The discovery of diverse codon reassignment events has demonstrated that the canonical genetic code is not universal. Studying coding reassignment at the molecular level is critical for understanding genetic code evolution, ...

    • Rational optimization of tolC as a powerful dual selectable marker for genome engineering 

      Gregg, Christopher J.; Lajoie, Marc J.; Napolitano, Michael G.; Mosberg, Joshua A.; Goodman, Daniel B.; Aach, John; Isaacs, Farren J.; Church, George M. (Oxford University Press, 2014)
      Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, ...