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Homology and Heterology Effects in Drosophila: Cohesin and Condensin as Chromosome Choreographers
(2016-05-02)
The organization of interphase nuclei has numerous consequences for gene expression and genome stability in eukaryotes. Here, we present studies of chromosome organization in Drosophila melanogaster over the course of the ...
Towards Sense Codon Reassignment in Escherichia Coli
(2015-10-23)
Expansion of the genetic code through engineering of the translation machinery has vastly increased the chemical repertoire of the proteome. Incorporation of non-canonical amino acids (ncAAs) entails use of engineered ...
A Gain of Function Senescence Bypass Screen Identifies the Homeobox Transcription Factor DLX2 as a Regulator of ATM-P53 Signaling
(2016-01-26)
Senescence stimuli activate multiple tumor suppressor pathways to initiate cycle arrest and a differentiation program characteristic of senescent cells. We performed a two-stage, gain-of function screen to select for the ...
Loss of the Histone Demethylase Phf8 Is Compatible With Development but Confers Resilience to Anxiety and Depression
(2016-01-12)
Phf8 is a histone demethylase associated with human developmental disorders and cancer. Early studies of Phf8 in humans indicated that inactivating mutations in the gene were linked to syndromic intellectual disability ...
Engineering of Allosteric Transcription Factors and Their Use for Metabolic Pathway Evolution
(2016-01-27)
Microbial metabolic production is an attractive alternative to traditional chemical synthesis for a wide array of commercially relevant molecules. Coaxing microbes to produce a target chemical efficiently often requires ...
Lineage and Functional Analyses of Specific Subsets of Retinal Progenitor Cells
(2015-05-21)
The vertebrate central nervous system (CNS) is made up of a diverse array of cell types. The retina, an accessible part of the CNS with seven major cell types and more than sixty cell subtypes, is an excellent model system ...
Estrogen Signaling in Breast Tumorigenesis
(2016-09-09)
The estrogen receptor (ER) is expressed in approximately 70% of sporadic breast cancers and is believed to activate genes driving tumorigenesis and initial cell proliferation. Therapeutic targeting of the ER signaling ...
The Regulation of RNA Polymerase II Transcription and Co-Transcriptional Processes
(2016-07-12)
Transcription by RNA polymerase II (Pol II) is one of the most highly regulated processes within the cell and is coupled to multiple co-transcriptional events. Furthermore, many different classes of coding and non-coding ...
An Anatomical and Functional Dissection of the Role Pet1 Raphe Neurons Play in Neonatal Cardiorespiratory Homeostasis
(2016-08-26)
Life-sustaining cardiorespiratory homeostasis requires the dynamic response of brainstem neural circuits. Apneas - the cessation of breathing often accompanied by bradycardia - can reflect defects in these circuits, and ...
A genetic dissection of the interactions between the CbtA toxin of Escherichia coli and the bacterial cytoskeleton
(2016-09-15)
Prokaryotic chromosomal toxin-antitoxin (TA) systems, consisting of a stable toxin and a labile, cotranscribed antitoxin, have been shown to target a number of essential processes in bacteria. The Escherichia coli genome ...