Browsing Harvard Medical School by Author "Li, Jianrong"
Now showing items 1-9 of 9
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A broad-spectrum antiviral targeting entry of enveloped viruses
Wolf, Mike C.; Freiberg, Alexander N.; Zhang, Tinghu; Akyol-Ataman, Zeynep; Grock, Andrew; Hong, Patrick W.; Li, Jianrong; Watson, Natalya F.; Fang, Angela Q.; Aguilar, Hector C.; Porotto, Matteo; Honko, Anna N.; Damoiseaux, Robert; Miller, John P.; Woodson, Sara E.; Chantasirivisal, Steven; Fontanes, Vanessa; Negrete, Oscar A.; Krogstad, Paul; Dasgupta, Asim; Moscona, Anne; Hensley, Lisa E.; Whelan, Sean P.; Faull, Kym F.; Holbrook, Michael R.; Jung, Michael E.; Lee, Benhur (National Academy of Sciences, 2010)We describe an antiviral small molecule, LJ001, effective against numerous enveloped viruses including Influenza A, filoviruses, poxviruses, arenaviruses, bunyaviruses, paramyxoviruses, flaviviruses, and HIV-1. In sharp ... -
A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping
Li, Jianrong; Rahmeh, Amal; Morelli, Marco; Whelan, Sean J. (American Society for Microbiology, 2008)Nonsegmented negative-sense (NNS) RNA viruses cap their mRNA by an unconventional mechanism. Specifically, 5 ' monophosphate mRNA is transferred to GDP derived from GTP through a reaction that involves a covalent intermediate ... -
A unique strategy for mRNA cap methylation used by vesicular stomatitis virus
Li, Jianrong; Wang, Jennifer T.; Whelan, Sean J. (National Academy of Sciences, 2006)Nonsegmented negative-sense (nsNS) RNA viruses typically replicate within the host cell cytoplasm and do not have access to the host mRNA capping machinery. These viruses have evolved a unique mechanism for mRNA cap formation ... -
Amino Acid Residues within Conserved Domain VI of the Vesicular Stomatitis Virus Large Polymerase Protein Essential for mRNA Cap Methyltransferase Activity
Li, Jianrong; Fontaine-Rodriguez, Errin C.; Whelan, Sean J. (American Society for Microbiology, 2005)During mRNA synthesis, the polymerase of vesicular stomatitis virus (VSV) copies the genomic RNA to produce five capped and polyadenylated mRNAs with the 5'-terminal structure 7(m)GpppA(m)pApCpApGpNpNpApUpCp. The 5' mRNA ... -
Mechanisms of Perinatal Brain Injury
Haynes, Robin Lynn; DeSilva, Tara M.; Li, Jianrong (Hindawi Publishing Corporation, 2012) -
mRNA Cap Methylation Influences Pathogenesis of Vesicular Stomatitis Virus in Vivo
Ma, Yuanmei; Wei, Yongwei; Zhang, Xiaodong; Zhang, Yu; Cai, Hui; Zhu, Yang; Shilo, Konstantin; Oglesbee, Michael; Krakowka, Steven; Whelan, Sean J.; Li, Jianrong (American Society for Microbiology, 2014)One role of mRNA cap guanine-N-7 (G-N-7) methylation is to facilitate the efficient translation of mRNA. The role of mRNA cap ribose 2'-O methylation is enigmatic, although recent work has implicated this as a signature ... -
Opposing Effects of Inhibiting Cap Addition and Cap Methylation on Polyadenylation during Vesicular Stomatitis Virus mRNA Synthesis
Li, Jianrong; Rahmeh, Amal; Brusic, Vesna; Whelan, Sean J. (American Society for Microbiology, 2009)The multifunctional large (L) polymerase protein of vesicular stomatitis virus (VSV) contains enzymatic activities essential for RNA synthesis, including mRNA cap addition and polyadenylation. We previously mapped amino ... -
Ribose 2′-O Methylation of the Vesicular Stomatitis Virus mRNA Cap Precedes and Facilitates Subsequent Guanine-N-7 Methylation by the Large Polymerase Protein
Rahmeh, Amal A.; Li, Jianrong; Kranzusch, Philip J.; Whelan, Sean J. (American Society for Microbiology, 2009)During conventional mRNA cap formation, two separate methyltransferases sequentially modify the cap structure, first at the guanine-N-7 (G-N-7) position and subsequently at the ribose 2'-O position. For vesicular stomatitis ... -
Vesicular Stomatitis Viruses Resistant to the Methylase Inhibitor Sinefungin Upregulate RNA Synthesis and Reveal Mutations That Affect mRNA Cap Methylation
Li, Jianrong; Chorba, John; Whelan, Sean (American Society for Microbiology, 2007)Sinefungin (SIN), a natural S-adenosyl-L-methionine analog produced by Streptomyces griseolus, is a potent inhibitor of methyltransferases. We evaluated the effect of SIN on replication of vesicular stomatitis virus (VSV), ...