Publication: Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor
No Thumbnail Available
Open/View Files
Date
2019-12-16
Published Version
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Science and Business Media LLC
The Harvard community has made this article openly available. Please share how this access benefits you.
Citation
Chen, Haiqi, Sophia Liu, Samuel Padula, Daniel Lesman, Kettner Griswold, Allen Lin, Tongtong Zhao, Jamie L. Marshall, and Fei Chen. 2020. “Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor.” Nature Biotechnology 38 (2): 165–68.
Research Data
Abstract
Here, we describe TRACE, a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. Using TRACE in a MEK1 inhibitor resistance screen, we identified functionally correlated mutations.
Description
Other Available Sources
Keywords
Biomedical Engineering, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology
Terms of Use
Metadata Only