dc.contributor.author | Liu, Xia | en_US |
dc.contributor.author | Busby, Jennifer | en_US |
dc.contributor.author | John, Ciny | en_US |
dc.contributor.author | Wei, Jianning | en_US |
dc.contributor.author | Yuan, Xin | en_US |
dc.contributor.author | Lu, Michael L. | en_US |
dc.date.accessioned | 2014-03-10T20:33:50Z | |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Liu, Xia, Jennifer Busby, Ciny John, Jianning Wei, Xin Yuan, and Michael L. Lu. 2013. “Direct Interaction between AR and PAK6 in Androgen-Stimulated PAK6 Activation.” PLoS ONE 8 (10): e77367. doi:10.1371/journal.pone.0077367. http://dx.doi.org/10.1371/journal.pone.0077367. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11878885 | |
dc.description.abstract | A p21-activated kinase 6 (PAK6) was previously identified to be an androgen receptor (AR) interacting protein through a yeast two-hybrid screening. We used hormone responsive prostate cancer LAPC4 and LNCap cell lines as models to study the signaling events associated with androgen stimulation and PAK6. An androgen-stimulated PAK6 kinase activation was observed in LAPC4 cells expressing endogenous PAK6 and in LNCap cells ectopically expressing a wild type PAK6. This activation was likely mediated through a direct interaction between AR and PAK6 since siRNA knock-down of AR in LAPC4 cells downregulated androgen-stimulated PAK6 activation. In addition, LNCap cells expressing a non-AR-interacting PAK6 mutant exhibited dampened androgen-stimulated kinase activation. As a consequence of androgen-stimulated activation, PAK6 was phosphorylated at multiple serine/threonine residues including the AR-interacting domain of PAK6. Furthermore, androgen-stimulation promoted prostate cancer cell motility and invasion were demonstrated in LNCap cells ectopically expressing PAK6-WT. In contrast, LNCap expressing non-AR-interacting mutant PAK6 did not respond to androgen stimulation with increased cell motility and invasion. Our results demonstrate that androgen-stimulated PAK6 activation is mediated through a direct interaction between AR and PAK6 and PAK6 activation promotes prostate cancer cells motility and invasion. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0077367 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795072/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Direct Interaction between AR and PAK6 in Androgen-Stimulated PAK6 Activation | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Yuan, Xin | en_US |
dc.date.available | 2014-03-10T20:33:50Z | |
dc.identifier.doi | 10.1371/journal.pone.0077367 | * |
dash.contributor.affiliated | Yuan, Xin | |