dc.contributor.author | Turetsky, Anna | en_US |
dc.contributor.author | Kim, Eunha | en_US |
dc.contributor.author | Kohler, Rainer H. | en_US |
dc.contributor.author | Miller, Miles A. | en_US |
dc.contributor.author | Weissleder, Ralph | en_US |
dc.date.accessioned | 2014-05-06T16:15:48Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Turetsky, Anna, Eunha Kim, Rainer H. Kohler, Miles A. Miller, and Ralph Weissleder. 2014. “Single cell imaging of Bruton's Tyrosine Kinase using an irreversible inhibitor.” Scientific Reports 4 (1): 4782. doi:10.1038/srep04782. http://dx.doi.org/10.1038/srep04782. | en |
dc.identifier.issn | 2045-2322 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12152828 | |
dc.description.abstract | A number of Bruton's tyrosine kinase (BTK) inhibitors are currently in development, yet it has been difficult to visualize BTK expression and pharmacological inhibition in vivo in real time. We synthesized a fluorescent, irreversible BTK binder based on the drug Ibrutinib and characterized its behavior in cells and in vivo. We show a 200 nM affinity of the imaging agent, high selectivity, and irreversible binding to its target following initial washout, resulting in surprisingly high target-to-background ratios. In vivo, the imaging agent rapidly distributed to BTK expressing tumor cells, but also to BTK-positive tumor-associated host cells. | en |
dc.language.iso | en_US | en |
dc.publisher | Nature Publishing Group | en |
dc.relation.isversionof | doi:10.1038/srep04782 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998017/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Single cell imaging of Bruton's Tyrosine Kinase using an irreversible inhibitor | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Scientific Reports | en |
dash.depositing.author | Turetsky, Anna | en_US |
dc.date.available | 2014-05-06T16:15:48Z | |
dc.identifier.doi | 10.1038/srep04782 | * |
dash.contributor.affiliated | Turetsky, Anna | |
dash.contributor.affiliated | Miller, Miles | |
dash.contributor.affiliated | Kim, Eunha | |
dash.contributor.affiliated | Kohler, Rainer | |
dash.contributor.affiliated | Weissleder, Ralph | |