dc.contributor.author | Jiang, Lin | en_US |
dc.contributor.author | Wallerman, Ola | en_US |
dc.contributor.author | Younis, Shady | en_US |
dc.contributor.author | Rubin, Carl-Johan | en_US |
dc.contributor.author | Gilbert, Elizabeth R. | en_US |
dc.contributor.author | Sundström, Elisabeth | en_US |
dc.contributor.author | Ghazal, Awaisa | en_US |
dc.contributor.author | Zhang, Xiaolan | en_US |
dc.contributor.author | Wang, Li | en_US |
dc.contributor.author | Mikkelsen, Tarjei S. | en_US |
dc.contributor.author | Andersson, Göran | en_US |
dc.contributor.author | Andersson, Leif | en_US |
dc.date.accessioned | 2014-05-06T16:17:08Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Jiang, L., O. Wallerman, S. Younis, C. Rubin, E. R. Gilbert, E. Sundström, A. Ghazal, et al. 2014. “ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells.” PLoS ONE 9 (4): e94187. doi:10.1371/journal.pone.0094187. http://dx.doi.org/10.1371/journal.pone.0094187. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12152887 | |
dc.description.abstract | ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0094187 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979763/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and life sciences | en |
dc.subject | Cell Biology | en |
dc.subject | Chromosome Biology | en |
dc.subject | Chromatin | en |
dc.subject | Computational Biology | en |
dc.subject | Genome Analysis | en |
dc.subject | Transcriptome Analysis | en |
dc.subject | Genome Expression Analysis | en |
dc.subject | Genetics | en |
dc.subject | Epigenetics | en |
dc.subject | Histone modification | en |
dc.subject | RNA interference | en |
dc.subject | Gene expression | en |
dc.subject | DNA transcription | en |
dc.subject | Gene regulation | en |
dc.subject | Genomics | en |
dc.subject | Functional Genomics | en |
dc.subject | Molecular Genetics | en |
dc.title | ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Mikkelsen, Tarjei S. | en_US |
dc.date.available | 2014-05-06T16:17:08Z | |
dc.identifier.doi | 10.1371/journal.pone.0094187 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Mikkelsen, Tarjei S. | |