Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

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Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

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Title: Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
Author: Dahlman, James E.; Abudayyeh, Omar O.; Joung, Julia; Gootenberg, Jonathan S.; Zhang, Feng; Konermann, Silvana

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Citation: Dahlman, James E., Omar O. Abudayyeh, Julia Joung, Jonathan S. Gootenberg, Feng Zhang, and Silvana Konermann. 2015. “Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease.” Nature biotechnology 33 (11): 1159-1161. doi:10.1038/nbt.3390. http://dx.doi.org/10.1038/nbt.3390.
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Abstract: We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct sgRNA constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14 15 bp target sequences and MS2 binding loops, can activate gene expression using an active Cas9 nuclease, without inducing DSBs. We use these ‘dead RNAs’ to perform orthogonal gene knockout and transcriptional activation in human cells.
Published Version: doi:10.1038/nbt.3390
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747789/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320383
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