Browsing Harvard Medical School by Title
Now showing items 82-101 of 18442
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A Drosophila Resource of Transgenic RNAi Lines for Neurogenetics
(Nature Research (part of Springer Nature), 2009)Conditional expression of hairpin constructs in Drosophila is a powerful method to disrupt the activity of single genes with a spatial and temporal resolution that. is impossible, or exceedingly difficult, using classical ... -
A Facile Coating Strategy to Functionalize Inorganic Nanoparticles for Biosensing
(American Chemical Society, 2017) -
A family of ParA-like ATPases promotes cell pole maturation by facilitating polar localization of chemotaxis proteins
(Cold Spring Harbor Laboratory Press, 2011)Stochastic processes are thought to mediate localization of membrane-associated chemotaxis signaling clusters in peritrichous bacteria. Here, we identified a new family of ParA-like ATPases (designated ParC [for partitioning ... -
A Forward Genetic Strategy Reveals Destabilizing Mutations in the Ebolavirus Glycoprotein That Alter Its Protease Dependence during Cell Entry
(American Society for Microbiology, 2010)Ebolavirus (EBOV) entry into cells requires proteolytic disassembly of the viral glycoprotein, GP. This proteolytic processing, unusually extensive for an enveloped virus entry protein, is mediated by cysteine cathepsins, ... -
A fragment of anthrax lethal factor delivers proteins to the cytosol without requiring protective antigen
(National Academy of Sciences, 2003)Anthrax protective antigen (PA) is a 735-aa polypeptide that facilitates the exit of anthrax lethal factor (LF) from the endosome to the cytosol where the toxin acts. We recently found, however, that a fusion protein of ... -
A Freeze Frame View of Vesicular Stomatitis Virus Transcription Defines a Minimal Length of RNA for 5′ Processing
(Public Library of Science, 2011)The RNA synthesis machinery of vesicular stomatitis virus (VSV) comprises the genomic RNA encapsidated by the viral nucleocapsid protein (N) and associated with the RNA dependent RNA polymerase, the viral components of ... -
A Functional Peroxisome Proliferator-activated Receptor-γ Ligand-binding Domain Is Not Required for Adipogenesis
(American Society for Biochemistry and Molecular Biology, 2008)The nuclear hormone receptor peroxisome proliferator-activated receptor-gamma ( PPAR gamma) is the central regulator of adipogenesis. Although it is the target for several drugs that function as agonist activators, a high ... -
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability
(Cold Spring Harbor Laboratory Press, 2010)In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to ... -
A Genome-Wide Gene Function Prediction Resource for Drosophila melanogaster
(Public Library of Science, 2010)Predicting gene functions by integrating large-scale biological data remains a challenge for systems biology. Here we present a resource for Drosophila melanogaster gene function predictions. We trained function-specific ... -
A genome-wide RNAi screen reveals multiple regulators of caspase activation
(Rockefeller University Press, 2007) -
A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
(American Society for Microbiology, 2015)Diverse environmental stimuli and a complex network of regulatory factors are known to modulate expression of Vibrio cholerae's principal virulence factors. However, there is relatively little known about how metabolic ... -
A Genome-Wide Small Interfering RNA Screen Identifies Host Factors Required for Vesicular Stomatitis Virus Infection
(American Society for Microbiology, 2014)Viruses are dependent on their host cells for replication and thus have evolved in intimate association with them. The identification of host factors required for viral infection has led to advances in both viral and ... -
A Genomewide RNA Interference Screen for Modifiers of Aggregates Formation by Mutant Huntingtin in Drosophila
(Nature Research (part of Springer Nature), 2010)Protein aggregates are a common pathological feature of most neurodegenerative diseases (NDs). Understanding their formation and regulation will help clarify their controversial roles in disease pathogenesis. To date, there ... -
A High Throughput Amenable Arabidopsis-P. aeruginosa System Reveals a Rewired Regulatory Module and the Utility to Identify Potent Anti-Infectives
(Public Library of Science, 2011)We previously demonstrated that in a metasystem consisting of Arabidopsis seedlings growing in liquid medium (in 96 well plates) even microbes considered to be innocuous such as laboratory strains of E. coli and B. subtilis ... -
A large conformational change of the translocation ATPase SecA
(National Academy of Sciences, 2004)The ATPase SecA mediates the posttranslational translocation of a wide range of polypeptide substrates through the SecY channel in the cytoplasmic membrane of bacteria. We have determined the crystal structure of a monomeric ... -
A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
(National Academy of Sciences, 2005)The advent of RNA interference has led to the ability to interfere with gene expression and greatly expanded our ability to perform genetic screens in mammalian cells. The expression of short hairpin RNA (shRNA) from ... -
A ligand-binding pocket in the dengue virus envelope glycoprotein
(National Academy of Sciences, 2003)Dengue virus is an emerging global health threat. Its major envelope glycoprotein, E, mediates viral attachment and entry by membrane fusion. A crystal structure of the soluble ectodomain of E from dengue virus type 2 ... -
A Light-activated Theranostic Nanoagent for Targeted Macrophage Ablation in Inflammatory Atherosclerosis
(Wiley, 2010)The synthesis and utility of a multimodal theranostic nanoagent based upon magnetofluorescent nanoparticles for the treatment of inflammatory atherosclerosis is described. These particles are modified with near-infrared ... -
A magneto-DNA nanoparticle system for rapid detection and phenotyping of bacteria
(Nature Research, 2013)So far, although various diagnostic approaches for pathogen detection have been proposed, most are too expensive, lengthy or limited in specificity for clinical use. Nanoparticle systems with unique material properties, ... -
A Metasystem of Framework Model Organisms to Study Emergence of New Host-Microbe Adaptations
(Public Library of Science, 2008)An unintended consequence of global industrialization and associated societal rearrangements is new interactions of microbes and potential hosts (especially mammals and plants), providing an opportunity for the rapid ...