Browsing HMS Scholarly Articles by Author "Bostina, Mihnea"
Now showing items 1-5 of 5
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An Interaction between DNA Polymerase and Helicase Is Essential for the High Processivity of the Bacteriophage T7 Replisome
Kulczyk, Arkadiusz W.; Akabayov, Barak; Lee, Seung-Joo; Bostina, Mihnea; Berkowitz, Steven A.; Richardson, Charles C. (American Society for Biochemistry and Molecular Biology, 2012)Synthesis of the leading DNA strand requires the coordinated activity of DNA polymerase and DNA helicase, whereas synthesis of the lagging strand involves interactions of these proteins with DNA primase. We present the ... -
Catching a Virus in the Act of RNA Release: a Novel Poliovirus Uncoating Intermediate Characterized by Cryo-Electron Microscopy
Levy, Hazel C.; Bostina, Mihnea; Filman, David J.; Hogle, James M. (American Society for Microbiology, 2010)Poliovirus infection requires that the particle undergo a series of conformational transitions that lead to cell entry and genome release. In an effort to understand the conformational changes associated with the release ... -
Mechanism of Action and Capsid-Stabilizing Properties of VHHs with an in Vitro Antipolioviral Activity
Schotte, Lise; Strauss, Mike; Thys, Bert; Halewyck, Hadewych; Filman, David J.; Bostina, Mihnea; Hogle, James M.; Rombaut, Bart (American Society for Microbiology, 2014)Previously, we reported on the in vitro antiviral activity of single-domain antibody fragments (VHHs) directed against poliovirus type 1. Five VHHs were found to neutralize poliovirus type 1 in an in vitro setting and ... -
Poliovirus RNA Is Released from the Capsid near a Twofold Symmetry Axis
Bostina, Mihnea; Levy, Hazel; Filman, David J.; Hogle, James M. (American Society for Microbiology, 2011)After recognizing and binding to its host cell, poliovirus (like other nonenveloped viruses) faces the challenge of translocating its genome across a cellular membrane and into the cytoplasm. To avoid entanglement with the ... -
RNA Transfer from Poliovirus 135S Particles across Membranes Is Mediated by Long Umbilical Connectors
Strauss, Mike; Levy, Hazel C.; Bostina, Mihnea; Filman, David J.; Hogle, James M. (American Society for Microbiology, 2013)During infection, the binding of poliovirus to its cell surface receptor at 37 degrees C triggers an expansion of the virus in which internal polypeptides that bind to membranes are externalized. Subsequently, in a poorly ...