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Chromatin Modification by PSC Occurs at One PSC per Nucleosome and Does Not Require the Acidic Patch of Histone H2A
(Public Library of Science, 2012)
Chromatin architecture is regulated through both enzymatic and non-enzymatic activities. For example, the Polycomb Group (PcG) proteins maintain developmental gene silencing using an array of chromatin-based mechanisms. ...
Gene-Drug Interactions and the Evolution of Antibiotic Resistance
(2013-03-18)
The evolution of antibiotic resistance is shaped by interactions between genes, the chemical environment, and an antibiotic's mechanism of action. This thesis explores these interactions with experiments, theory, and ...
Genetic Characterization of smg-8 Mutants Reveals No Role in C. elegans Nonsense Mediated Decay
(Public Library of Science, 2012)
The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use ...
Phenotypes and genetic mechanisms of C. elegans enhanced RNAi
(2013-10-08)
RNA interference (RNAi) potently and specifically induces gene knockdown, and its potential for reverse genetics in Caenorhabditis elegans is enormous. However, even in these nematodes, RNAi can be induced more effectively ...
GW8510 Increases Insulin Expression in Pancreatic Alpha Cells through Activation of p53 Transcriptional Activity
(Public Library of Science, 2012)
Background: Expression of insulin in terminally differentiated non-beta cell types in the pancreas could be important to treating type-1 diabetes. Previous findings led us to hypothesize involvement of kinase inhibition ...
Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi
(Public Library of Science, 2016)
Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the ...
Angiotensin II Induced Cardiac Dysfunction on a Chip
(Public Library of Science, 2016)
In vitro disease models offer the ability to study specific systemic features in isolation to better understand underlying mechanisms that lead to dysfunction. Here, we present a cardiac dysfunction model using angiotensin ...
Identification of Listeria monocytogenes Determinants Required for Biofilm Formation
(Public Library of Science, 2014)
Listeria monocytogenes is a Gram-positive, food-borne pathogen of humans and animals. L. monocytogenes is considered to be a potential public health risk by the U.S. Food and Drug Administration (FDA), as this bacterium ...
SiteOut: An Online Tool to Design Binding Site-Free DNA Sequences
(Public Library of Science, 2016)
DNA-binding proteins control many fundamental biological processes such as transcription, recombination and replication. A major goal is to decipher the role that DNA sequence plays in orchestrating the binding and activity ...
Inhibition of IKKα by BAY61-3606 Reveals IKKα-Dependent Histone H3 Phosphorylation in Human Cytomegalovirus Infected Cells
(Public Library of Science, 2016)
Protein kinase inhibitors can be used as tools to identify proteins and pathways required for virus replication. Using virus replication assays and western blotting we found that the widely used protein kinase inhibitor ...