dc.contributor.author | Yardimci, Hasan | en_US |
dc.contributor.author | Wang, Xindan | en_US |
dc.contributor.author | Loveland, Anna B. | en_US |
dc.contributor.author | Tappin, Inger | en_US |
dc.contributor.author | Rudner, David Z. | en_US |
dc.contributor.author | Hurwitz, Jerard | en_US |
dc.contributor.author | van Oijen, Antoine M. | en_US |
dc.contributor.author | Walter, Johannes C. | en_US |
dc.date.accessioned | 2014-02-13T19:01:43Z | |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Yardimci, Hasan, Xindan Wang, Anna B. Loveland, Inger Tappin, David Z. Rudner, Jerard Hurwitz, Antoine M. van Oijen, and Johannes C. Walter. 2012. “Bypass of a protein roadblock by a replicative DNA helicase.” Nature 492 (7428): 205-209. doi:10.1038/nature11730. http://dx.doi.org/10.1038/nature11730. | en |
dc.identifier.issn | 0028-0836 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:11708610 | |
dc.description.abstract | Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (“steric exclusion”). In contrast, large T antigen (T-ag), the replicative DNA helicase of the Simian Virus 40 (SV40), is reported to function as a pair of stacked hexamers that pumps double-stranded DNA through its central channel while laterally extruding single-stranded DNA. Here, we use single-molecule and ensemble assays to show that T-ag assembled on the SV40 origin unwinds DNA efficiently as a single hexamer that translocates on single-stranded DNA in the 3′ to 5′ direction. Unexpectedly, T-ag unwinds DNA past a DNA-protein crosslink on the translocation strand, suggesting that the T-ag ring can open to bypass bulky adducts. Together, our data underscore the profound conservation among replicative helicase mechanisms while revealing a new level of plasticity in their interactions with DNA damage. | en |
dc.language.iso | en_US | en |
dc.relation.isversionof | doi:10.1038/nature11730 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521859/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Bypass of a protein roadblock by a replicative DNA helicase | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Nature | en |
dash.depositing.author | Wang, Xindan | en_US |
dc.date.available | 2014-02-13T19:01:43Z | |
dc.identifier.doi | 10.1038/nature11730 | * |
dash.contributor.affiliated | Loveland, Anna Barbara | |
dash.contributor.affiliated | Wang, Xindan | |
dash.contributor.affiliated | Rudner, David | |