Segregation of molecules at cell division reveals native protein localization

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Segregation of molecules at cell division reveals native protein localization

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Title: Segregation of molecules at cell division reveals native protein localization
Author: Landgraf, Dirk; Okumus, Burak; Chien, Peter; Baker, Tania A.; Paulsson, Johan

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Citation: Landgraf, Dirk, Burak Okumus, Peter Chien, Tania A. Baker, and Johan Paulsson. 2013. “Segregation of molecules at cell division reveals native protein localization.” Nature methods 9 (5): 480-482. doi:10.1038/nmeth.1955. http://dx.doi.org/10.1038/nmeth.1955.
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Abstract: We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used fluorescent proteins (FPs) cause severe mislocalization when fused to homo-oligomers. Re-tagging five other reportedly foci-forming proteins with the most monomeric FP tested suggests the foci were caused by the FPs.
Published Version: doi:10.1038/nmeth.1955
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779060/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11877087
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