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Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters

dc.contributor.authorBhattacharyya, Sancharien_US
dc.contributor.authorBershtein, Shimonen_US
dc.contributor.authorShakhnovich, Eugene Ien_US
dc.date.accessioned2017-12-06T16:25:00Z
dc.date.issued2017en_US
dc.identifier.citationBhattacharyya, Sanchari, Shimon Bershtein, and Eugene I Shakhnovich. 2017. “Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters.” Bio-protocol 7 (14): e2396. doi:10.21769/BioProtoc.2396. http://dx.doi.org/10.21769/BioProtoc.2396.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34493370
dc.description.abstractThis protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.21769/BioProtoc.2396en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697766/pdf/en
dash.licenseLAAen_US
dc.subjectGene-dosage toxicityen
dc.subjectArabinoseen
dc.subjectProtein abundanceen
dc.subjectWestern-bloten
dc.subjectFitnessen
dc.subjectOver-expressionen
dc.subjectPlasmiden
dc.titleGene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promotersen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalBio-protocolen
dash.depositing.authorBhattacharyya, Sancharien_US
dc.date.available2017-12-06T16:25:00Z
dc.identifier.doi10.21769/BioProtoc.2396*
dash.contributor.affiliatedBhattacharyya, Sanchari
dash.contributor.affiliatedShakhnovich, Eugene


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