Browsing FAS Theses and Dissertations by Author "Church, George M"
Now showing items 1-8 of 8
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Accelerating the Understanding and Design of Intracellular Biosensors by Massively Multiplexed Experimentation and Machine Learning
Garruss, Alexander Shineman (2021-01-06)Recent progress in large-scale DNA synthesis and next-generation DNA sequencing technology have enabled studies of biological processes at a massive scale. These studies can be further coupled to advanced computational ... -
AGE REVERSAL BY TRANSCRIPTOMIC REPROGRAMMING
Plesa, Alexandru (2022-11-23)Aging is a progressive multifaceted functional decline of a biological system. Chronic age-related conditions such as cancer, cardiovascular, and neurodegenerative diseases are leading causes of death worldwide. As the ... -
Applications of recombineering and recoded genome construction.
Rudolph, Alexandra Isla (2022-06-06)Genome engineering offers the opportunity to bypass natural selection to generate novel organisms. Although multiple types of genome engineering are currently possible, work described in this dissertation occurred in the ... -
Development of Bioinformatics Solutions for Single-cell Transcriptomics and Novel Codec System for Robust DNA-based Data Storage
Zhou, Guangyu (2020-11-23)With the unprecedented wealth of biological and biomedical data generated, bioinformatics has shaped our understandings in life science through various applications: from the de novo assembly to pathway network analysis ... -
Factors affecting AAV production: Mutagenesis of AAV2 rep and AAV transgenes
Jain, Nina Kumari (2024-01-09)Recombinant adeno-associated viruses (rAAVs) are the predominant gene therapy vector. Previous work in the AAV field focused on capsid engineering to enable targeting of vectors to specific tissues and has resulted in five ... -
High throughput functional variant screens via in-vivo production of single-stranded DNA
Schubert, Max Gabriel (2020-11-23)Tremendous genetic variation exists in nature, but our ability to create and characterize individual genetic variants remains far more limited in scale. Likewise, engineering proteins and phenotypes requires the introduction ... -
Synthetic Genome Construction Techniques to Edit the Genetic Code of Escherichia coli
Baas-Thomas, Maximilien Sinclair (2022-11-23)The expansion of the genetic code to allow for site-specific incorporation of non-standard amino acids (nsAAs) — proteogenic amino acids other than the 22 found across life — is an important step in the development of new ... -
Towards a Non-Invasive Model for Women’s Uterine Health: From Transcriptional Programs to Tissue Assembly
Huang, Jeremy Yuenchen (2021-07-12)In human females of reproductive age, the uterine endometrium undergoes a cyclic process of hormone-induced rapid cellular differentiation, enhanced vascular development, matrix remodeling, and structural change. Functional ...